Sh104-060908-WS

A synthetic peptide to phospho Y683 & Y684 of rat TrkA (and the corresponding phosphorylated sequences in TrkB and TrkC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse, human, chicken and zebrafish. Additionally, the antigen shares ~90% identity with the corresponding phosphorylated sequences in TrkB and TrkC.

TRKA_RAT
TRKA_MOUSE
TRKA_HUMAN
TRKA_CHICKEN
TRKA_DANIO RERIO

High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor

FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF.
TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Meakin S.O, et al. Proc. Natl. Acad. Sci. U.S.A. 89:2374-2378(1992).
2. Wooten M.W, et al. J. Biol. Chem. 276:7709-7712(2001).

For research use only

	Code Product Name OST00252G Sheep pan antibody to phospho Y683 & Y684 of TrkA, B, C

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. This antibody recognises also the corresponding phosphorylated sequences in TrkB and TrkC.

Specific for TrkA, TrkB, TrkC.

Rat, mouse, human. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

- Click to download the Data Sheet

- Click to download the Material Safety Data Sheet