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Code
OST00245G
$260USD
ID Tag
Rb128-281007-G
Immunogen
A synthetic peptide from the 4th cytoplasmic loop of human TRPM7 (LTRPC7) conjugated to blue carrier protein was used as the antigen.
Accession
Also known
Transient receptor potential cation channel subfamily M member 7, long transient receptor potential channel 7, LTrpC7, channel-kinase 1, CHAK1, LTRPC7, CHAK, FLJ20117, FLJ25718, TRP-PLIK
Target
TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein.
COFACTOR: Binds 1 zinc ion per subunit.
SUBUNIT: Homodimer. Forms heterodimers with TRPM6.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein.
COFACTOR: Binds 1 zinc ion per subunit.
SUBUNIT: Homodimer. Forms heterodimers with TRPM6.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ryazanova L.V, et al. J. Biol. Chem. 279:3708-3716(2004).
Limitation
For research use only
Related
products
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Code
OST00245G
$260USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB (confirmed by recombinant protein). A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for TRPM7.
Spcs X-react.
Human, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OST00245G
$260USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00245G
$260USD
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IF on PC12 cells at a concentration of 30 µg/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG (OST00245G), DAPI counterstained. |
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IF on PC12 cells at a concentration of 30 µg/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG (OST00245G), DAPI counterstained appearing in blue. |
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