Rb730-221108-WS

A synthetic peptide from the extracellular domain of rat amiloride sensitive sodium channel subunit gamma (SCNN1G, SCNEG, Gamma-ENaC) conjugated to immunogenic carrier protein was used as the immunogen. The antigen shares 94% identity with mouse's.

Amiloride-sensitive sodium channel subunit gamma (SCNN1G, SCNEG), Epithelial Na(+) channel subunit gamma, Gamma-ENaC, Gamma-NaCH, PHA1, ENaCg, SCNEG, ENaCgamma

FUNCTION: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2.
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note: Apical membrane of epithelial cells.
PTM: Phosphorylated on serine and threonine residues.
PTM: Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation.
DISEASE: Defects in SCNN1G are a cause of Liddle syndrome. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Canessa C.M, et al. Nature 367:463-467(1994)
2. Lingueglia R, et al. J. Biol. Chem. 269:13736-13739(1994)
3. Kreutz R, et al. Hypertension 29:131-136(1997)
4. Staub O, et al. EMBO J. 16:6325-6336(1997)
5. Shimkets R.A, et al. Proc. Natl. Acad. Sci. U.S.A. 95:3301-3305(1998)

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specific for SCNN1G.

Rat. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

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