GAPDH antibody

OVERVIEW
SPECS
CONTROL PEPTIDE
MSDS/DS
IMAGES

Code

OSG00035G

$240USD

ID Tag

Sh101-060908-G

Immunogen

A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Accession

GAPDH_RAT
GAPDH_MOUSE
GAPDH_HUMAN

Also known

38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase

Target

FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date

12 months after reconstitution

Shipping

This item will be shipped to you at ambient temperature in a lyophilised form.

References

1. Fort P, et al. Nucleic Acids Res. 13:1431-1442(1985).
2. Ballif B.A, et al. J. Proteome Res. 7:311-318(2008).

Limitation

For research use only

Related
products

Code	Product Name
OSG00034W	Sheep antibody to GAPDH

Code

OSG00035G

$240USD

Unit size

500 Âµg

Conjugate

Unconjugated antibody

Host

Sheep

Purity

IgG

Clonality

Polyclonal

Isotype

Polyclonal, IgG

Applications

IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity

Specific for GAPDH.

Spcs X-react.

Rat, mouse, human. Other species not yet tested.

Format

Lyophilised

Reconstitution

Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code

OSG00035G