⚬ Services
Code
OSG00032W
$240USD    Buy Now 

ID Tag
Rb659-060908-WS

Immunogen
A synthetic peptide from rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.

Accession

Also known
38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase

Target
FUNCTION: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Fort P, et al. Nucleic Acids Res. 13:1431-1442(1985).

Limitation
For research use only

Related
products

Code
OSG00032W
$240USD    Buy Now 

Unit size
150 ul

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
WB. A dilution of 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for GAPDH.

Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.

Format
Lyophilised. This product contains 0.02% benzalkonium chloride.

Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSG00032W
$240USD    Buy Now 


Rabbit antibody to GAPDH WB on different cell lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.

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Rabbit antibody to GAPDH
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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