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Code
OSS00098G
$240USD
ID Tag
Rb3243-120518-WS
Immunogen
A synthetic peptide from human SNIP conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Accession
Also known
p130 Cas associated protein, p140 Cap, SNAP 25 interacting protein, P140
Target
FUNCTION: Delays the onset of cell spreading in the early stages of cell adhesion to fibronectin. Also involved in calcium-dependent exocytosis from PC12 cells.
SUBUNIT: Interacts with the N-terminal coiled-coil region of SNAP25. Interacts with BCAR1/p130Cas through its C-terminal domain.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note: Localized to the perinuclear region, lamellopodia, cortical actin and actin stress fibers but not to focal adhesions.
SUBUNIT: Interacts with the N-terminal coiled-coil region of SNAP25. Interacts with BCAR1/p130Cas through its C-terminal domain.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note: Localized to the perinuclear region, lamellopodia, cortical actin and actin stress fibers but not to focal adhesions.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ota T, et al. Nat. Genet. 36:40-45(2004).
Limitation
For research use only
Related
products
products
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Code
OSS00098G
$240USD
Unit size
500 ug
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for SNIP.
Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSS00098G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSS00098G
$240USD
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IHC-P on paraffin sections of rat brain.
The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.
Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.
Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses.
Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer.
Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. |
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