Rb693-060908-WS

A synthetic peptide from the n-terminal of human CHMP4B, C conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.

CHMP4B_HUMAN
CHMP4C_HUMAN

CHMP4B: Chromatin-modifying protein 4b, Vacuolar protein-sorting-associated protein 7-2, SNF7-2, hSnf7-2, SNF7 homolog associated with Alix 1, hVps32, SHAX1, Charged multivesicular body protein 4b. CHMP4C: Chromatin modifying protein 4c, Vacuolar protein-sorting-associated protein 7-3, SNF7-3, hSnf7-3, SNF7 homolog associated with Alix 3, SHAX3, Charged multivesicular body protein 4c

FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: CHMP4B: Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.
TISSUE SPECIFICITY: CHMP4C: Expressed in heart, spleen and kidney.
DISEASE: Defects in CHMP4B are the cause of posterior polar cataract type 3 (CTPP3). Cataract is the most frequent cause of visual impairment and blindness worldwide. Posterior polar cataract is a distinctive opacity located at the back of the lens. Because of its proximity to the optical center of the eye, posterior polar cataract can have a marked effect on visual acuity.
MISCELLANEOUS: Its overexpression strongly inhibits HIV-1 release.

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

12 months after reconstitution

This item will be shipped to you at ambient temperature in a lyophilised form.

1. Katoh K, et al. J. Biol. Chem. 278:39104-39113(2003).
2. Deloukas P, et al. Nature 414:865-871(2001).

For research use only

100 µl

Unconjugated antibody

NZ white rabbit

Whole serum

Polyclonal

Polyclonal, whole serum

IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Specific for CHMP4B, C.

Mouse, rat, human. Other species not yet tested.

Lyophilised

Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

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