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Code
OSL00004G
$240USD
ID Tag
Sh103-060908-G
Immunogen
A synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse.
Accession
Also known
LIM domain kinase 2, LIMK-2
Target
FUNCTION: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
TISSUE SPECIFICITY: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
TISSUE SPECIFICITY: Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Okano I, et al. J. Biol. Chem. 270:31321-31330(1995).
2. Sumi T, et al. J. Biol. Chem. 276:670-676(2001).
2. Sumi T, et al. J. Biol. Chem. 276:670-676(2001).
Limitation
For research use only
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Code
OSL00004G
$240USD
Unit size
500 µg
Conjugate
Unconjugated antibody
Host
Sheep
Purity
IgG
Clonality
Polyclonal
Isotype
Polyclonal, IgG
Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.
Specificity
Specific for LIMK2.
Spcs X-react.
Mouse, rat, human. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSL00004G
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
⚬ Products
Sheep antibody to phospho S291 & S293 LIMK2
