⚬ Services
Code
OSN00008W
$240USD    Buy Now 

ID Tag
Rb666-060908-WS

Immunogen
A synthetic peptide from the WW1 domain of mouse NEDD4 conjugated to blue carrier protein was used as the antigen. The antigen shares 96% identity with human NEDD4.

Accession

Also known
Neural precursor cell expressed developmentally down-regulated protein 4, E3 ubiquitin-protein ligase NEDD4, NEDD-4

Target
FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
FUNCTION: Involved in the embryonic development and differentiation of the central nervous system.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Kumar S, et al. Biochem. Biophys. Res. Commun. 185:1155-1161(1992).
2. Hatakeyama S, et al. J. Biol. Chem. 272:15085-15092(1997).
3. Carninci P, et al. Science 309:1559-1563(2005).

Limitation
For research use only

Code
OSN00008W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for NEDD4.

Spcs X-react.
Mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Rabbit antibody to NEDD4
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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