Code
OSN00008W
$240USD
ID Tag
Rb666-060908-WS
Immunogen
A synthetic peptide from the WW1 domain of mouse NEDD4 conjugated to blue carrier protein was used as the antigen. The antigen shares 96% identity with human NEDD4.
Accession
Also known
Neural precursor cell expressed developmentally down-regulated protein 4, E3 ubiquitin-protein ligase NEDD4, NEDD-4
Target
FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
FUNCTION: Involved in the embryonic development and differentiation of the central nervous system.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Brain.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Kumar S, et al. Biochem. Biophys. Res. Commun. 185:1155-1161(1992).
2. Hatakeyama S, et al. J. Biol. Chem. 272:15085-15092(1997).
3. Carninci P, et al. Science 309:1559-1563(2005).
Limitation
For research use only
Code
OSN00008W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for NEDD4.
Spcs X-react.
Mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.