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Code
OSC00049W
$240USD
ID Tag
Rb653-060908-WS
Immunogen
A synthetic peptide from the c-terminal region of mouse CHMP6 conjugated to an antigen carrier protein was used as the antigen. The antigen shares 95% identity with rat's.
Accession
Also known
Charged multivesicular body protein 6, Chromatin-modifying protein 6
Target
FUNCTION: Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-II complex on endosomal membranes.
SUBUNIT: Component of the endosomal sorting required for transport complex III (ESCRT-III), at least composed of CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3/VPS24, CHMP4A/SHAX2, CHMP4B/SHAX1, CHMP4C/SHAX3, CHMP5/SNF7DC2 and CHMP6/VPS20. In the complex, it interacts directly with CHMP4A, CHMP4B and CHMP4C. Interacts directly with SNF8, VPS25 and VPS36 components of the ESCRT-II complex.
SUBCELLULAR LOCATION: Intracytoplasmic membrane. Endosome membrane; Lipid-anchor . Note: Localizes to endosomal membranes.
SUBUNIT: Component of the endosomal sorting required for transport complex III (ESCRT-III), at least composed of CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3/VPS24, CHMP4A/SHAX2, CHMP4B/SHAX1, CHMP4C/SHAX3, CHMP5/SNF7DC2 and CHMP6/VPS20. In the complex, it interacts directly with CHMP4A, CHMP4B and CHMP4C. Interacts directly with SNF8, VPS25 and VPS36 components of the ESCRT-II complex.
SUBCELLULAR LOCATION: Intracytoplasmic membrane. Endosome membrane; Lipid-anchor . Note: Localizes to endosomal membranes.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Watahiki A, et al. Nat. Methods 1 (3), 233-239(2004).
Limitation
For research use only
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Code
OSC00049W
$240USD
Unit size
100 ul
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for CHMP6.
Spcs X-react.
Mouse, rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSC00049W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSC00049W
$240USD
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WB on rat brain lysate under reducing/denatured conditions using Rabbit antibody to CHMP6 (OSC00049W) at 1 : 1000 dilution. Absorption of the antibody with the immunising peptide completely abolishes the detected band. Note: the expected MW is 23.5 kD however, a band of ~70 kD is detected. This might be due to CHMP6 being a component of a bigger complex. |
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