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IHC on paraffin sections of rat DRG tissue. The animal was perfused using Autoperfuser at 110 mmHg with 300 ml of 4%FA and further post-fixed overnight. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
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IHC on paraffin sections of rat DRG tissue. The animal was perfused using Autoperfuser at 110 mmHg with 300 ml of 4%FA and further post-fixed overnight. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |