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Code
OSR00302W
$240USD
ID Tag
Sh109-060908-WS
Immunogen
A synthetic peptide from human Rho-related GTP-binding protein RhoB (ARH6, ARHB, H6) conjugated to an immunogenic carrier protein was used as the antigen.
Accession
Also known
H6, ARH6, ARHB, RHOH6, MST081, MSTP081
Target
FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN.
SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation.
SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN.
SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
INDUCTION: By UV irradiation, N-methyl-N-nitrosourea, cisplatin, cyclohexamide and serum stimulation.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Nakamura T, et al. Biochem. Biophys. Res. Commun. 226:688-694(1996).
2. Westmark C.J, et al. Oncogene 24:502-511(2005).
3. Carninci P, et al. cience 309:1559-1563(2005).
2. Westmark C.J, et al. Oncogene 24:502-511(2005).
3. Carninci P, et al. cience 309:1559-1563(2005).
Limitation
For research use only
Related
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Code
OSR00302W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
Sheep
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for RhOB.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OSR00302W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
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Sheep antibody to RhoB
