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Code
OST00185W
$240USD
ID Tag
Rb621-170808-WS
Immunogen
A synthetic peptide from the 4th cytoplasmic loop of rat TRPV4 (VRL-2, VRL2) conjugated to an immunogenic carrier protein was used as the immunogen.
The location of the epitope is highlighted in green.
The location of the epitope is highlighted in green.
Accession
Also known
Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-related, smotically-activated channel, VR-OAC, Vroac, Otrpc4
Target
TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.
FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.
SIMILARITY: Belongs to the transient receptor family, TrpV subfamily.
SIMILARITY: Contains 3 ANK repeats.
FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.
SIMILARITY: Belongs to the transient receptor family, TrpV subfamily.
SIMILARITY: Contains 3 ANK repeats.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Liedtke W.B, et al. Cell 103:525-535(2000).
2. Marrelli,S.P, et al. Am. J. Physiol. Heart Circ. Physiol. 292 (3), H1390-H1397 (2007).
2. Marrelli,S.P, et al. Am. J. Physiol. Heart Circ. Physiol. 292 (3), H1390-H1397 (2007).
Limitation
For research use only
Code
OST00185W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1: 300 to 1: 3000 is recommended. The optimal dilution should be determined by the end user.
Specificity
Specific for TRPV4.
Spcs X-react.
Rat. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
Code
OST00185W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00185W
$240USD
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to TRPV4: OST00185W. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat kidney tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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IHC on paraffin sections of rat testis tissue using Rabbit antibody to TRPV4: OST00185W. HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin. |
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