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Code
OSV00017G
$240USD    Buy Now 

ID Tag
Rb451-150608-G

Immunogen
A synthetic peptide from rat VPS45A conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous with the corresponding sequence in mouse.

Accession

Also known
Vps45, VPS45, rvps45, Vps45a

Target
FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network.
SUBUNIT: Interacts with STX6 and ZFYVE20.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network.
TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. El-Husseini A.E.-D, et al. Biochim. Biophys. Acta 1325:8-12(1997).
2. Tellam J.T, et al. J. Biol. Chem. 272:6187-6193(1997).

Limitation
For research use only

Related
products
 
Code Product Name
OSV00016W Rabbit antibody to VPS45A
 

Code
OSV00017G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Specificity
Specific for VPS45.

Spcs X-react.
Rat, mouse. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSV00017G
$240USD    Buy Now 


Rabbit antibody to VPS45A IHC on rat spinal cord using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to VPS45A IHC on rat spinal cord using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to VPS45A IHC on rat spinal cord using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to VPS45A IHC on rat brain using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to VPS45A IHC on rat spinal cord using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Rabbit antibody to VPS45A IHC on rat brain using Rabbit antibody to VPS45A (OSV00017G) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
⚬ Products
Rabbit antibody to VPS45A
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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