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Code
OSV00014W
$240USD
ID Tag
Rb470-150608-WS
Immunogen
A synthetic peptide from aa region 50-100 of human VAMPB conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including human, rat, mouse, zebra fish, bovine, xenopus and chicken.
Accession
Also known
VAMP-associated protein B, VAMP-B, VAP-B, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C, VAMP-B/VAMP-C, VAP-B/VAP-C, VAPB, VAMP A
Target
FUNCTION: May play a role in vesicle trafficking.
SUBUNIT: Homodimer, and heterodimer with VAPA. Interacts with VAMP1 and VAMP2.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type IV membrane protein. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
TISSUE SPECIFICITY: Ubiquitous. Isoform 1 predominates.
DISEASE: Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
DISEASE: Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy; also called late-onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late- adult-onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
SUBUNIT: Homodimer, and heterodimer with VAPA. Interacts with VAMP1 and VAMP2.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type IV membrane protein. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
TISSUE SPECIFICITY: Ubiquitous. Isoform 1 predominates.
DISEASE: Defects in VAPB are the cause of amyotrophic lateral sclerosis type 8 (ALS8). ALS8 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
DISEASE: Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy; also called late-onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late- adult-onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Nishimura Y, et al. Biochem. Biophys. Res. Commun. 254:21-26(1999).
Limitation
For research use only
Related
products
products
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Code
OSV00014W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 2000 to 1 : 3000 is recommended for WB and 1:1000 to 1:2000 for IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Specificity
Specific for VAMP A and B.
Spcs X-react.
Human, rat, mouse. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com
- Click to see the eLab Book
Code
OSV00014W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OSV00014W
$240USD
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IHC on free floating sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for OCT embedding. Tissues were cut at 30 um thickness. Primary antibody: dilution 1: 2000, incubated overnight. |
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WB on rat tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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IHC on free floating sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for OCT embedding. Tissues were cut at 30 um thickness. Primary antibody: dilution 1: 2000, incubated overnight. |
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IHC on free floating sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for OCT embedding. Tissues were cut at 30 um thickness. Primary antibody: dilution 1: 2000, incubated overnight. |
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IHC on free floating sections of rat spinal cord. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for OCT embedding. Tissues were cut at 30 um thickness. Primary antibody: dilution 1: 2000, incubated overnight. |
⚬ Products
Rabbit antibody to VAMP A/B (50-100)
