⚬ Services
Code
OSS00069G
$240USD    Buy Now 

ID Tag
Sh88-290608-G

Immunogen
A synthetic peptide from mouse SNAP-25 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Accession

Also known
SNAP-25, Synaptosomal-associated 25 kDa protein, SNAP25, RIC4, SEC9, SNAP, RIC-4, Super protein, SUP

Target
FUNCTION: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1, SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.
SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation.
TISSUE SPECIFICITY: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Madison D.L, et al. J. Neurochem. 72:988-998(1999).

Limitation
For research use only

Related
products
 
Code Product Name
OSS00068W Sheep antibody to SNAP-25
 

Code
OSS00069G
$240USD    Buy Now 

Unit size
500 µg

Conjugate
Unconjugated antibody

Host
Sheep

Purity
IgG

Clonality
Polyclonal

Isotype
Polyclonal, IgG

Applications
IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Specificity
Specific for SNAP-25.

Spcs X-react.
Rat, mouse, human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

⚬ Products
Sheep antibody to SNAP-25
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides